Isoform-specific O-GlycosylationofOsteopontinand ...
2020-12-1 · bone powder was suspended in 20 to 30 volumes of 4 M guani-dine HCl, 50 mM Tris-HCl (pH 7.4), ... blocked with 5% nonfat dry milk for 1 h, followed by incubation with primary antibodies 2A1(1/1000)for1h,orwithanti-bonesialoproteinII(1/1000) ... mented with 10% fetal bovine serum (Invitrogen) and trans-fected with pF1-mT3 (ppGalNacT-3) or vector ...
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