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  • Bovine superoxide dismutase: A radioimmunoassay ...

    1978-10-1 · Rabbit antibodies to bovine superoxide dismutase have been produced and used to develop a double-antibody solid phase radioimmunoassay for the enzyme. The assay is sensitive and highly specific for the bovine enzyme, showing no cross-reactivity with the murine or human superoxide …

    Get Price
  • Variation of Superoxide Dismutase in Bovine Milk ...

    1978-8-1 · All cows were of good health and on normal feed but varied in stage of lactation and age. Average superoxide dismutase concentration was 1.1 ± .48 units/ml with a range of .06 to 2.88 units/ml and a variance of .23. Concentration of superoxide dismutase in milk was approximately 10 2 lower than in bovine blood.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    Superoxide dismutase has been purified from bovine heart by a procedure which was previously developed for the isola- tion of this enzyme from bovine erythrocytes.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    1971-5-10 · The superoxide dismutase from bovine erythrocytes and from bovine heart were accordingly analyzed for zinc by the dithizone method (14). The erythrocyte enzyme was found to contain 1.71 and the heart enzyme 1.76 moles of zinc per 32,600 g of enzyme.

    Get Price
  • Superoxide dismutase of bovine and frog rod outer …

    1975-12-1 · Bovine rod outer segments (ROS) contain soluble superoxide dismutase (SOD) which from cyanide sensitivity and electrophoretic mobility appears identical to Cu Zn SOD of erythrocytes. Enzyme activity of ROS extracts is 200–400 times as much as remainder of retina.

    Get Price
  • Superoxide dismutase ameliorates neuronal death from ...

    Superoxide Dismutase Ameliorates Neuronal Death From Hypoxia in Culture Daniel M. Rosenbaum, MD; Jacqueline Kalberg, BA; John A. Kessler, MD Background and Purpose Studies showing efficacy with free radical scavengers have been conflicting, and when protection was demonstrated it was attributed to action at the level of the vascular endothelium.

    Get Price
  • THE EFFECTS OF SUPEROXIDE DISMUTASE KNOCKOUT …

    2017-8-28 · Superoxide dismutase (CuZnSOD, SOD1) is another fundamental antioxidant enzyme of the red blood cell, and is the only form of SOD present in mature mammalian erythrocytes. It has been found that Sod1-/-mice have a reduced lifespan with indices of oxidative stress in their blood such as increased serum iron and decreased plasma ascorbate [6-8].

    Get Price
  • Adriamycin stimulated superoxide formation in ...

    Adriamycin (doxorubicin), an anticancer agent, stimulated the formation of superoxide in submitochondrial particles isolated from bovine heart. Superoxide formation was detected by oxygen uptake, by the cooxidation of epinephrine to adrenochrome and by the reduction of acetylated cytochrome c.

    Get Price
  • A rapid whole-cell assay for superoxide dismutase ...

    1982-3-15 · ANALYTICAL BIOCHEMISTRY 121, 207-212 (1982) A Rapid Whole-Cell Assay for Superoxide Dismutase GARRY C. WHITELAM AND GEOFFREY A. CODD Department of Biological Sciences, University of Dundee, Dundee, DDI 4HN, United Kingdom Received August 31, 1981 A procedure is described for the intact-cell assay of superoxide dismutase(s).

    Get Price
  • Superoxide Dismutase Enzymosomes: Carrier Capacity ...

    2014-7-19 · Purpose A strategy not usually used to improve carrier-mediated delivery of therapeutic enzymes is the attachment of the enzymes to the outer surface of liposomes. The aim of our work was to design a new type of enzymosomes with a sufficient surface-exposed enzyme load while preserving the structural integrity of the liposomal particles and activity of the enzyme. Methods The therapeutic ...

    Get Price
  • Bovine superoxide dismutase: A radioimmunoassay ...

    1978-10-1 · Rabbit antibodies to bovine superoxide dismutase have been produced and used to develop a double-antibody solid phase radioimmunoassay for the enzyme. The assay is sensitive and highly specific for the bovine enzyme, showing no cross-reactivity with the murine or human superoxide …

    Get Price
  • Variation of Superoxide Dismutase in Bovine Milk ...

    1978-8-1 · All cows were of good health and on normal feed but varied in stage of lactation and age. Average superoxide dismutase concentration was 1.1 ± .48 units/ml with a range of .06 to 2.88 units/ml and a variance of .23. Concentration of superoxide dismutase in milk was approximately 10 2 lower than in bovine blood.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    Superoxide dismutase has been purified from bovine heart by a procedure which was previously developed for the isola- tion of this enzyme from bovine erythrocytes.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    1971-5-10 · The superoxide dismutase from bovine erythrocytes and from bovine heart were accordingly analyzed for zinc by the dithizone method (14). The erythrocyte enzyme was found to contain 1.71 and the heart enzyme 1.76 moles of zinc per 32,600 g of enzyme.

    Get Price
  • Superoxide dismutase of bovine and frog rod outer …

    1975-12-1 · Bovine rod outer segments (ROS) contain soluble superoxide dismutase (SOD) which from cyanide sensitivity and electrophoretic mobility appears identical to Cu Zn SOD of erythrocytes. Enzyme activity of ROS extracts is 200–400 times as much as remainder of retina.

    Get Price
  • Superoxide dismutase ameliorates neuronal death from ...

    Superoxide Dismutase Ameliorates Neuronal Death From Hypoxia in Culture Daniel M. Rosenbaum, MD; Jacqueline Kalberg, BA; John A. Kessler, MD Background and Purpose Studies showing efficacy with free radical scavengers have been conflicting, and when protection was demonstrated it was attributed to action at the level of the vascular endothelium.

    Get Price
  • THE EFFECTS OF SUPEROXIDE DISMUTASE KNOCKOUT …

    2017-8-28 · Superoxide dismutase (CuZnSOD, SOD1) is another fundamental antioxidant enzyme of the red blood cell, and is the only form of SOD present in mature mammalian erythrocytes. It has been found that Sod1-/-mice have a reduced lifespan with indices of oxidative stress in their blood such as increased serum iron and decreased plasma ascorbate [6-8].

    Get Price
  • Adriamycin stimulated superoxide formation in ...

    Adriamycin (doxorubicin), an anticancer agent, stimulated the formation of superoxide in submitochondrial particles isolated from bovine heart. Superoxide formation was detected by oxygen uptake, by the cooxidation of epinephrine to adrenochrome and by the reduction of acetylated cytochrome c.

    Get Price
  • A rapid whole-cell assay for superoxide dismutase ...

    1982-3-15 · ANALYTICAL BIOCHEMISTRY 121, 207-212 (1982) A Rapid Whole-Cell Assay for Superoxide Dismutase GARRY C. WHITELAM AND GEOFFREY A. CODD Department of Biological Sciences, University of Dundee, Dundee, DDI 4HN, United Kingdom Received August 31, 1981 A procedure is described for the intact-cell assay of superoxide dismutase(s).

    Get Price
  • Superoxide Dismutase Enzymosomes: Carrier Capacity ...

    2014-7-19 · Purpose A strategy not usually used to improve carrier-mediated delivery of therapeutic enzymes is the attachment of the enzymes to the outer surface of liposomes. The aim of our work was to design a new type of enzymosomes with a sufficient surface-exposed enzyme load while preserving the structural integrity of the liposomal particles and activity of the enzyme. Methods The therapeutic ...

    Get Price
  • Superoxide Dismutase Assay Kit II

    The Calbiochem ® Superoxide Dismutase Assay Kit II utilizes a tetrazolium salt for detection of superoxide radicals generated by xanthine oxidase and hypoxanthine. One unit of SOD is defined as the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical. The SOD assay measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD).

    Get Price
  • Adriamycin stimulated superoxide formation in ...

    Adriamycin (doxorubicin), an anticancer agent, stimulated the formation of superoxide in submitochondrial particles isolated from bovine heart. Superoxide formation was detected by oxygen uptake, by the cooxidation of epinephrine to adrenochrome and by the reduction of acetylated cytochrome c.

    Get Price
  • Blood replacement with nanobiotechnologically …

    Unlike donor red blood cells (RBCs), blood substitutes can be treated to remove infective agents and can be used on the spot or in the ambulance in emergency without the time-consuming typing and cross-matching. Donor RBC requires storage at 4 degrees and is only good for 42 days, but blood substitu …

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  • Extraction of Erythrocyte Enzymes for the Preparation of ...

    2018-5-28 · Briefly, fresh bovine blood with heparin as an anticoagulant was centrifuged at 4000 g for 60 minutes at 48C. The plasma supernatant and the upper layer of the red blood cell pellet that contains the buffy coat were removed. The sedimented red blood cells were washed four times with sterile, ice-cold 0.9% NaCl. Red blood cells were then ...

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  • Superoxide Dismutase Activity in Leukocytes

    2014-1-30 · Assay for superoxide dismutase: epinephrine procedure. Superoxide dismutase activity was detected by its ability to inhibit the autoxidation of epinephrine at pH 10.2 as described by Misra and Fridovich (12). Each cuvette con- tained in a final volume of 3.0 ml: 0.50 ml of 1.8 mM epinephrine (freshly prepared); 0.50 ml of 0.6 mMEDTA; 0.50 ml of 0.30 Msodium carbonate, pH 10.2, and leuko-

    Get Price
  • Oxidative Stress-Induced Sirtuin1 Downregulation ...

    2021-4-7 · Al-Khaldi A., Sultan S. The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism. BMC. Endocr. Disord. 2019; 19:19. doi: 10.1186/s12902-019-0350-y. [PMC free article] [Google Scholar]

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  • Superoxide dismutase composition for periodontal use ...

    3. A composition according to claim 1, wherein said superoxide dismutase is bovine superoxide dismutase. 4. A composition according to claim 1, wherein the amount of superoxide dismutase is within the range of from about 1×10-4 weight percent to about …

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  • The respiratory burst of bovine neutrophilis

    Eur. J. Biochem 152,669-679 (1985) ( FEBS 1985 The respiratory burst of bovine neutrophils Role of a b type cytochrome and coenzyme specificity FranCoise MOREL, Jacques DOUSSIERE, Marie-Jose STASIA and Pierre V. VICNAlS Laboratoire de Biochimie, Dtpartement de Recherche Fondamentale, Centre &Etudes Nuclkaires, Grcnoble (Received May 7/July 11, 1985)

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  • Acceptability of blood and blood substitutes - Ferguson ...

    2008-1-17 · Data already published shows that people perceive blood substitutes as riskier than donor blood, with bovine haemoglobin‐based substitutes perceived as the most risky [5, 6, 33-35; see Ref. 1 for a review], and the results reported here on a large sample confirm this. Furthermore, the results reported here show that perceptions of risk and ...

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  • Trace Minerals and Livestock: Not Too Much Not Too Little

    The new approaches of the animal production systems make managing the mineral nutrition a challenge. Versus the excessive, trace mineral supply in intensively managed livestock, well above the physiological requirements, is the no trace mineral supplementation of organic systems, which become highly dependent on trace minerals in the soil. Nowadays, in addition to the animal health perspective ...

    Get Price
  • Bovine superoxide dismutase: A radioimmunoassay ...

    1978-10-1 · Rabbit antibodies to bovine superoxide dismutase have been produced and used to develop a double-antibody solid phase radioimmunoassay for the enzyme. The assay is sensitive and highly specific for the bovine enzyme, showing no cross-reactivity with the murine or human superoxide …

    Get Price
  • Variation of Superoxide Dismutase in Bovine Milk ...

    1978-8-1 · All cows were of good health and on normal feed but varied in stage of lactation and age. Average superoxide dismutase concentration was 1.1 ± .48 units/ml with a range of .06 to 2.88 units/ml and a variance of .23. Concentration of superoxide dismutase in milk was approximately 10 2 lower than in bovine blood.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    Superoxide dismutase has been purified from bovine heart by a procedure which was previously developed for the isola- tion of this enzyme from bovine erythrocytes.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    1971-5-10 · The superoxide dismutase from bovine erythrocytes and from bovine heart were accordingly analyzed for zinc by the dithizone method (14). The erythrocyte enzyme was found to contain 1.71 and the heart enzyme 1.76 moles of zinc per 32,600 g of enzyme.

    Get Price
  • Superoxide dismutase of bovine and frog rod outer …

    1975-12-1 · Bovine rod outer segments (ROS) contain soluble superoxide dismutase (SOD) which from cyanide sensitivity and electrophoretic mobility appears identical to Cu Zn SOD of erythrocytes. Enzyme activity of ROS extracts is 200–400 times as much as remainder of retina.

    Get Price
  • Superoxide dismutase ameliorates neuronal death from ...

    Superoxide Dismutase Ameliorates Neuronal Death From Hypoxia in Culture Daniel M. Rosenbaum, MD; Jacqueline Kalberg, BA; John A. Kessler, MD Background and Purpose Studies showing efficacy with free radical scavengers have been conflicting, and when protection was demonstrated it was attributed to action at the level of the vascular endothelium.

    Get Price
  • THE EFFECTS OF SUPEROXIDE DISMUTASE KNOCKOUT …

    2017-8-28 · Superoxide dismutase (CuZnSOD, SOD1) is another fundamental antioxidant enzyme of the red blood cell, and is the only form of SOD present in mature mammalian erythrocytes. It has been found that Sod1-/-mice have a reduced lifespan with indices of oxidative stress in their blood such as increased serum iron and decreased plasma ascorbate [6-8].

    Get Price
  • Adriamycin stimulated superoxide formation in ...

    Adriamycin (doxorubicin), an anticancer agent, stimulated the formation of superoxide in submitochondrial particles isolated from bovine heart. Superoxide formation was detected by oxygen uptake, by the cooxidation of epinephrine to adrenochrome and by the reduction of acetylated cytochrome c.

    Get Price
  • A rapid whole-cell assay for superoxide dismutase ...

    1982-3-15 · ANALYTICAL BIOCHEMISTRY 121, 207-212 (1982) A Rapid Whole-Cell Assay for Superoxide Dismutase GARRY C. WHITELAM AND GEOFFREY A. CODD Department of Biological Sciences, University of Dundee, Dundee, DDI 4HN, United Kingdom Received August 31, 1981 A procedure is described for the intact-cell assay of superoxide dismutase(s).

    Get Price
  • Superoxide Dismutase Enzymosomes: Carrier Capacity ...

    2014-7-19 · Purpose A strategy not usually used to improve carrier-mediated delivery of therapeutic enzymes is the attachment of the enzymes to the outer surface of liposomes. The aim of our work was to design a new type of enzymosomes with a sufficient surface-exposed enzyme load while preserving the structural integrity of the liposomal particles and activity of the enzyme. Methods The therapeutic ...

    Get Price
  • Bovine superoxide dismutase: A radioimmunoassay ...

    1978-10-1 · Rabbit antibodies to bovine superoxide dismutase have been produced and used to develop a double-antibody solid phase radioimmunoassay for the enzyme. The assay is sensitive and highly specific for the bovine enzyme, showing no cross-reactivity with the murine or human superoxide …

    Get Price
  • Variation of Superoxide Dismutase in Bovine Milk ...

    1978-8-1 · All cows were of good health and on normal feed but varied in stage of lactation and age. Average superoxide dismutase concentration was 1.1 ± .48 units/ml with a range of .06 to 2.88 units/ml and a variance of .23. Concentration of superoxide dismutase in milk was approximately 10 2 lower than in bovine blood.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    Superoxide dismutase has been purified from bovine heart by a procedure which was previously developed for the isola- tion of this enzyme from bovine erythrocytes.

    Get Price
  • Further Characterization of Bovine Superoxide Dismutase ...

    1971-5-10 · The superoxide dismutase from bovine erythrocytes and from bovine heart were accordingly analyzed for zinc by the dithizone method (14). The erythrocyte enzyme was found to contain 1.71 and the heart enzyme 1.76 moles of zinc per 32,600 g of enzyme.

    Get Price
  • Superoxide dismutase of bovine and frog rod outer …

    1975-12-1 · Bovine rod outer segments (ROS) contain soluble superoxide dismutase (SOD) which from cyanide sensitivity and electrophoretic mobility appears identical to Cu Zn SOD of erythrocytes. Enzyme activity of ROS extracts is 200–400 times as much as remainder of retina.

    Get Price
  • Superoxide dismutase ameliorates neuronal death from ...

    Superoxide Dismutase Ameliorates Neuronal Death From Hypoxia in Culture Daniel M. Rosenbaum, MD; Jacqueline Kalberg, BA; John A. Kessler, MD Background and Purpose Studies showing efficacy with free radical scavengers have been conflicting, and when protection was demonstrated it was attributed to action at the level of the vascular endothelium.

    Get Price
  • THE EFFECTS OF SUPEROXIDE DISMUTASE KNOCKOUT …

    2017-8-28 · Superoxide dismutase (CuZnSOD, SOD1) is another fundamental antioxidant enzyme of the red blood cell, and is the only form of SOD present in mature mammalian erythrocytes. It has been found that Sod1-/-mice have a reduced lifespan with indices of oxidative stress in their blood such as increased serum iron and decreased plasma ascorbate [6-8].

    Get Price
  • Adriamycin stimulated superoxide formation in ...

    Adriamycin (doxorubicin), an anticancer agent, stimulated the formation of superoxide in submitochondrial particles isolated from bovine heart. Superoxide formation was detected by oxygen uptake, by the cooxidation of epinephrine to adrenochrome and by the reduction of acetylated cytochrome c.

    Get Price
  • A rapid whole-cell assay for superoxide dismutase ...

    1982-3-15 · ANALYTICAL BIOCHEMISTRY 121, 207-212 (1982) A Rapid Whole-Cell Assay for Superoxide Dismutase GARRY C. WHITELAM AND GEOFFREY A. CODD Department of Biological Sciences, University of Dundee, Dundee, DDI 4HN, United Kingdom Received August 31, 1981 A procedure is described for the intact-cell assay of superoxide dismutase(s).

    Get Price
  • Superoxide Dismutase Enzymosomes: Carrier Capacity ...

    2014-7-19 · Purpose A strategy not usually used to improve carrier-mediated delivery of therapeutic enzymes is the attachment of the enzymes to the outer surface of liposomes. The aim of our work was to design a new type of enzymosomes with a sufficient surface-exposed enzyme load while preserving the structural integrity of the liposomal particles and activity of the enzyme. Methods The therapeutic ...

    Get Price