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  • Embryo number and periconceptional undernutrition in …

    2014-7-15 · Exposure to poor maternal nutrition around the time of conception results in an early prepartum activation of the fetal pituitary-adrenal axis and in increased adrenal growth and stress response after birth associated with epigenetic changes in a differentially methylated region (DMR) of adrenal IGF2/H19.We have determined the effects of maternal undernutrition during the …

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  • Maternal nutrient restriction between early-to-mid ...

    Introduction. The incidence of obesity (BMI >30 kg/m 2) in the UK population and worldwide has increased markedly over the past decade (1, 2).Many epidemiological and prospective clinical studies in humans, together with experimental findings in animals, have demonstrated that modifications of the nutritional environment during fetal development can have a substantial impact on later health ...

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  • Comparison of 2, 5, and 20 % O2 on the development of …

    2016-3-23 · Purpose The objective of this study is to investigate the effect of 2, 5, and 20 % O2 on post-thaw day 3 human embryo culture until blastocyst stage. Methods One hundred fifty-five day 3 human embryos were used. One hundred twenty out of 155 embryos were recovered after thawing. Surviving embryos were distributed into 2, 5, or 20 % O2 groups and cultured for 2.5 days. At the end of culture ...

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  • Effect of Slow-Release Pellets of Selenium and Iodine on ...

    2019-8-7 · The need for minerals during pregnancy in mammals increases by progress of gestation due to embryo development. Therefore, the supply of trace minerals for pregnant ewes in late pregnancy may be of vital importance. The present study was carried out to evaluate the effect of selenium and iodine release slowly on the performance and some blood parameters of pregnant ewes and their lambs ...

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  • effects of chemical and physical factors on mammalian ...

    The supply of high levels of protein or urea (2.5%) during peri-conception increases levels of ammonium in plasma (150 µM) and the reproductive tract (McEvoy et al., 1997; Gardner et al., 2004), thus creating an environment that is disruptive to embryo development in vivo.

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  • Engineered heart tissue for regeneration of diseased ...

    2004-4-1 · Engineered heart tissue (EHT) is made from a liquid cell matrix-mixture composed of neutralized, liquid collagen type I, extracellular matrix proteins (Matrigel ® ), and freshly isolated heart cells from neonatal rats. This reconstitution mixture can …

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  • STEMCELL Technologies

    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

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  • Amino Acids in the Production of Chicken Egg and Muscle ...

    The analyses of muscle tissue indicate amino acid requirements for growth different from those for egg production (Table 3). The higher contents of arginine, histidine, lysine and threonine in chicken muscle, over those of the egg, indicate a relatively greater need for these amino acids during the period of muscle formation, or growth.

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  • Comparative analysis of differentially expressed genes ...

    2019-5-6 · A multitude of genes tightly regulate ovarian follicular development and hormone secretion. These complex and coordinated biological processes are altered during pregnancy. In order to further understand the regulatory role of these genes during pregnancy, it is important to screen the differentially expressed genes (DEGs) in the ovaries of pregnant and nonpregnant mammals.

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  • (PDF) Fixation of carbon dioxide by preimplantation rabbit ...

    During the first 3 days of development of the rabbit embryo, carbon fixed from CO 2 accumulated in the embryos and culture medium and increased as development proceeded.

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  • Brilliant Cresyl Blue stain selects largest oocytes with ...

    2011-10-1 · Embryo development of prepubertal sheep oocytes selected with different BCB concentrations. The percentage of BCB+ obtained after staining with different concentrations of BCB was 19, 28, 36 and 47% for 13, 26, 39 and 52 μM BCB respectively ().Although staining with 13 μM BCB showed a low percentage of stained oocytes (BCB+), the number of blastocysts obtained in this group …

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  • CSIRO PUBLISHING

    2018-8-9 · This article is a compilation of pieces that are part biographical sketches and part personal recollections of 18 scientists with whom the author was acquainted in three continents over almost 50 years. The subjects, from Australia, the United States and the United Kingdom, will be recognisable to many in the field, especially more experienced scientists. For younger scientists, the article ...

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  • BRAC Artificial Insemination Enterprise

    2020-8-19 · BRAC Artificial Insemination Enterprise. BRAC's artificial insemination initiative began as a partnering initiative with the Bangladesh government's livestock department in 1987. The aim was to provide people from low-income groups in rural areas with access to better quality cattle breeds. While BRAC provided cattle as assets to such ...

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  • Alginate: properties and biomedical applications

    2. Alginate: general properties. Commercially available alginate is typically extracted from brown algae (Phaeophyceae), including Laminaria hyperborea, Laminaria digitata, Laminaria japonica, Ascophyllum nodosum, and Macrocystis pyrifera [] by treatment with aqueous alkali solutions, typically with NaOH [].The extract is filtered, and either sodium or calcium chloride is added to the filtrate ...

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  • Exploring dry storage as an alternative biobanking ...

    2019-3-1 · Probably the first reported attempt to apply drying to vertebrate cells was the partial drying performed by Polge, Smith and Parkes [].In that study they diluted 1 mL of fowl semen with 1 mL of 20–30% glycerol in Ringer's solution, froze it to −79 °C, warmed it back to …

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  • Catalogue for Guiding Industry Restructuring (2019 Edition)

    2021-2-8 · 57. Application of WDGS and liquid organic fertilizer II. Water conservancy 1. Construction of rivers, lakes and sea dikes and river regulation projects 2. Cross-basin water transfer projects 3. Urban and rural water supply and source projects 4. Rural drinking water safety projects 5. Construction of flood storage and detention area 6.

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  • STEMCELL Technologies

    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

    Get Price
  • Current and Past Strategies for Bacterial Culture in ...

    2021-8-27 · INTRODUCTION. As proposed by Robert Koch, a pure culture is the foundation of all research in infectious diseases (1, 2).The first isolation of a bacterium enables the design of experimental models to analyze virulence and to complete Koch's criteria, thereby establishing a link between microorganisms and infectious diseases ().Bacterial culture also enables the study of the antibiotic ...

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  • (PDF) MANUAL ON HOUSING FOR PIGS

    Pigs housed in a single pen system show about 2% better growth rate and 13% more efficient feed utilization than pigs that change pens in the normal fashion. Also, there are fewer cases of tail biting and cannibalism, while pens need not to be disinfected as often. The system also requires less labour.

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  • Artificial Insemination in Pigs- A Step by Step Guide ...

    2018-1-30 · Insert the tip of the rod at a slight upward angle to avoid hitting the urethral opening. Once the tip of the rod is in, continue to gently press the rod in until you feel the resistance of the cervix, then begin twisting the rod gently counter-clockwise. Once the rod is in place, you can attach the bag of semen to the end of the rod.

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  • Embryo Thawing - ART Reproductive Center

    Embryo thawing is the procedure to recover previously frozen embryos prior to an embryo transfer. After the embryo is retrieved from liquid nitrogen storage and all patient identifiers are checked, it is placed in a prepared dish and processed through several drops of thaw materials.

    Get Price
  • Method 533: Determination of Per- and Polyfluoroalkyl ...

    2021-6-25 · 533-1 . 1 Scope and Application . This is a solid phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of select per- and polyfluoroalkyl substances (PFAS) in drinking water.

    Get Price
  • Extraction of DNA for Plant Leaves / Leaf / Embryo / Seeds ...

    DNA Extraction. 1. Preheat 5ml CTAB (add 10µl mercaptoethanol to each 5ml CTAB) in a blue-topped 50ml centrifuge tube at 60-65 o C. Remove and discard midribs, and wrap laminae in aluminium foil and freeze in liquid nitrogen. 0.5 – 1.0 gm tissue/5ml CTAB. (Can store leaf material after liquid Nitrogen – 1-2 days at –20 or –80 for ...

    Get Price
  • An automatic and sensitive method for simultaneous ...

    2016-8-31 · An automatic and sensitive method for simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 4 derivatives by online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography (UHPLC) with online solvent filtration in groundwater samples was developed. Ground

    Get Price
  • Embryo number and periconceptional undernutrition in …

    2014-7-15 · Exposure to poor maternal nutrition around the time of conception results in an early prepartum activation of the fetal pituitary-adrenal axis and in increased adrenal growth and stress response after birth associated with epigenetic changes in a differentially methylated region (DMR) of adrenal IGF2/H19.We have determined the effects of maternal undernutrition during the …

    Get Price
  • (PDF) DNA Extraction Protocol without Liquid Nitrogen of ...

    2016-2-18 · DNA Extraction Protocol without Liquid Nitrogen of Cold Tolerant Rice ... When DNA migrated sufficiently the power supply was ... The remnant half seeds containing the embryo …

    Get Price
  • Frontiers

    2019-7-3 · The first commercially available gonadotropin product was a human chorionic gonadotropin (hCG) extract, followed by animal pituitary gonadotropin extracts. These extracts were effective, leading to the introduction of the two-step protocol, which involved ovarian stimulation using animal gonadotropins followed by ovulation triggering using hCG. However, ovarian response to animal …

    Get Price
  • Novel generation of deep eutectic solvent as an acceptor ...

    2018-2-1 · Stock solutions 1.0 mg mL −1 were prepared by dissolving each analyte in HPLC-grade methanol that was stored at 4 °C. The mixtures of the diluted standard solutions were daily prepared in ultra-pure water. 2.2. Chromatographic apparatus. The chromatographic equipment was an Agilent 1260 HPLC system, contained a quaternary pump, degasser, and UV–vis detector (Waldbronn, Germany).

    Get Price
  • A Guide to Forest Seed Handling - Home

    2021-2-17 · A. Add 1 kg animal repellent (endrin) to 1 kg bird repellent (thiram). B. Stir. C. Beat with paint stirrer. D. Add 700 ml latex adhesive to another 1 kg bird repellent (thiram) E. Blend by pouring from can to can about 10 times. F. Pour finished repellent directly onto 10 kg of seed as concrete mixer is turning. Tumble about 2 minutes. 8.11 The U.S. Forest Service recommendation for preparing ...

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  • PROCESS FOR THE EXTRACTION OF ENZYMES FROM …

    The extraction procedure of the invention will be illustrated further. Enzymes of fungi and bacteria can be extracted using urea solutions. The cells obtained by liquid culture are collected by centrifuge or filtration, washed with water and used in the subsequent procedures.

    Get Price
  • Embryo Thawing - ART Reproductive Center

    Embryo thawing is the procedure to recover previously frozen embryos prior to an embryo transfer. After the embryo is retrieved from liquid nitrogen storage and all patient identifiers are checked, it is placed in a prepared dish and processed through several drops of thaw materials.

    Get Price
  • Method 533: Determination of Per- and Polyfluoroalkyl ...

    2021-6-25 · 533-1 . 1 Scope and Application . This is a solid phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of select per- and polyfluoroalkyl substances (PFAS) in drinking water.

    Get Price
  • Extraction of DNA for Plant Leaves / Leaf / Embryo / Seeds ...

    DNA Extraction. 1. Preheat 5ml CTAB (add 10µl mercaptoethanol to each 5ml CTAB) in a blue-topped 50ml centrifuge tube at 60-65 o C. Remove and discard midribs, and wrap laminae in aluminium foil and freeze in liquid nitrogen. 0.5 – 1.0 gm tissue/5ml CTAB. (Can store leaf material after liquid Nitrogen – 1-2 days at –20 or –80 for ...

    Get Price
  • An automatic and sensitive method for simultaneous ...

    2016-8-31 · An automatic and sensitive method for simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 4 derivatives by online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography (UHPLC) with online solvent filtration in groundwater samples was developed. Ground

    Get Price
  • Embryo number and periconceptional undernutrition in …

    2014-7-15 · Exposure to poor maternal nutrition around the time of conception results in an early prepartum activation of the fetal pituitary-adrenal axis and in increased adrenal growth and stress response after birth associated with epigenetic changes in a differentially methylated region (DMR) of adrenal IGF2/H19.We have determined the effects of maternal undernutrition during the …

    Get Price
  • (PDF) DNA Extraction Protocol without Liquid Nitrogen of ...

    2016-2-18 · DNA Extraction Protocol without Liquid Nitrogen of Cold Tolerant Rice ... When DNA migrated sufficiently the power supply was ... The remnant half seeds containing the embryo …

    Get Price
  • Frontiers

    2019-7-3 · The first commercially available gonadotropin product was a human chorionic gonadotropin (hCG) extract, followed by animal pituitary gonadotropin extracts. These extracts were effective, leading to the introduction of the two-step protocol, which involved ovarian stimulation using animal gonadotropins followed by ovulation triggering using hCG. However, ovarian response to animal …

    Get Price
  • Novel generation of deep eutectic solvent as an acceptor ...

    2018-2-1 · Stock solutions 1.0 mg mL −1 were prepared by dissolving each analyte in HPLC-grade methanol that was stored at 4 °C. The mixtures of the diluted standard solutions were daily prepared in ultra-pure water. 2.2. Chromatographic apparatus. The chromatographic equipment was an Agilent 1260 HPLC system, contained a quaternary pump, degasser, and UV–vis detector (Waldbronn, Germany).

    Get Price
  • A Guide to Forest Seed Handling - Home

    2021-2-17 · A. Add 1 kg animal repellent (endrin) to 1 kg bird repellent (thiram). B. Stir. C. Beat with paint stirrer. D. Add 700 ml latex adhesive to another 1 kg bird repellent (thiram) E. Blend by pouring from can to can about 10 times. F. Pour finished repellent directly onto 10 kg of seed as concrete mixer is turning. Tumble about 2 minutes. 8.11 The U.S. Forest Service recommendation for preparing ...

    Get Price
  • PROCESS FOR THE EXTRACTION OF ENZYMES FROM …

    The extraction procedure of the invention will be illustrated further. Enzymes of fungi and bacteria can be extracted using urea solutions. The cells obtained by liquid culture are collected by centrifuge or filtration, washed with water and used in the subsequent procedures.

    Get Price
  • Embryo Thawing - ART Reproductive Center

    Embryo thawing is the procedure to recover previously frozen embryos prior to an embryo transfer. After the embryo is retrieved from liquid nitrogen storage and all patient identifiers are checked, it is placed in a prepared dish and processed through several drops of thaw materials.

    Get Price
  • Method 533: Determination of Per- and Polyfluoroalkyl ...

    2021-6-25 · 533-1 . 1 Scope and Application . This is a solid phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of select per- and polyfluoroalkyl substances (PFAS) in drinking water.

    Get Price
  • Extraction of DNA for Plant Leaves / Leaf / Embryo / Seeds ...

    DNA Extraction. 1. Preheat 5ml CTAB (add 10µl mercaptoethanol to each 5ml CTAB) in a blue-topped 50ml centrifuge tube at 60-65 o C. Remove and discard midribs, and wrap laminae in aluminium foil and freeze in liquid nitrogen. 0.5 – 1.0 gm tissue/5ml CTAB. (Can store leaf material after liquid Nitrogen – 1-2 days at –20 or –80 for ...

    Get Price
  • An automatic and sensitive method for simultaneous ...

    2016-8-31 · An automatic and sensitive method for simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 4 derivatives by online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography (UHPLC) with online solvent filtration in groundwater samples was developed. Ground

    Get Price
  • Embryo number and periconceptional undernutrition in …

    2014-7-15 · Exposure to poor maternal nutrition around the time of conception results in an early prepartum activation of the fetal pituitary-adrenal axis and in increased adrenal growth and stress response after birth associated with epigenetic changes in a differentially methylated region (DMR) of adrenal IGF2/H19.We have determined the effects of maternal undernutrition during the …

    Get Price
  • (PDF) DNA Extraction Protocol without Liquid Nitrogen of ...

    2016-2-18 · DNA Extraction Protocol without Liquid Nitrogen of Cold Tolerant Rice ... When DNA migrated sufficiently the power supply was ... The remnant half seeds containing the embryo …

    Get Price
  • Frontiers

    2019-7-3 · The first commercially available gonadotropin product was a human chorionic gonadotropin (hCG) extract, followed by animal pituitary gonadotropin extracts. These extracts were effective, leading to the introduction of the two-step protocol, which involved ovarian stimulation using animal gonadotropins followed by ovulation triggering using hCG. However, ovarian response to animal …

    Get Price
  • Novel generation of deep eutectic solvent as an acceptor ...

    2018-2-1 · Stock solutions 1.0 mg mL −1 were prepared by dissolving each analyte in HPLC-grade methanol that was stored at 4 °C. The mixtures of the diluted standard solutions were daily prepared in ultra-pure water. 2.2. Chromatographic apparatus. The chromatographic equipment was an Agilent 1260 HPLC system, contained a quaternary pump, degasser, and UV–vis detector (Waldbronn, Germany).

    Get Price
  • A Guide to Forest Seed Handling - Home

    2021-2-17 · A. Add 1 kg animal repellent (endrin) to 1 kg bird repellent (thiram). B. Stir. C. Beat with paint stirrer. D. Add 700 ml latex adhesive to another 1 kg bird repellent (thiram) E. Blend by pouring from can to can about 10 times. F. Pour finished repellent directly onto 10 kg of seed as concrete mixer is turning. Tumble about 2 minutes. 8.11 The U.S. Forest Service recommendation for preparing ...

    Get Price
  • PROCESS FOR THE EXTRACTION OF ENZYMES FROM …

    The extraction procedure of the invention will be illustrated further. Enzymes of fungi and bacteria can be extracted using urea solutions. The cells obtained by liquid culture are collected by centrifuge or filtration, washed with water and used in the subsequent procedures.

    Get Price